RNA

Part:BBa_K4061041:Design

Designed by: Diya Agrawal   Group: iGEM21_HKUST   (2021-10-15)


Antinsense RNA against Constitutive eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The antisense mRNA anneals 22nt on the protein CDS as well as binds to the RBS region on the mRNA and also the -10 promoter region. On the 3' end we have also encoded an Hfq-binding spot which enhances regulatory RNA stability. The Hfq protein is native to E. coli.


Source

Benchling-DNA self-designed. Ordered via IDT

References